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Genetic resistances to plant viruses and their vectors

Bibliographic Reference from
Virology Department PPI - PPI - NCRZ
Kostinbrod - Bulgaria

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Last database update for these data: 2007-08-29 - Data extracted on 2009-04-14 from the database.

Differentiation of Bulgarian Isolates of Cucumber Mosaic Virus by Serological Methods

Bibliographic Reference
Field Value
Document typeJournal article: paper
LanguageEnglish
Year2002
AuthorsHristova, D.
Yordanova, A.
Mavrodieva,V.
TitleDifferentiation of Bulgarian Isolates of Cucumber Mosaic Virus by Serological Methods
SourceJournal of Phytopathology
Volume150
Pages334-339
AbstractAn extended study was carried out on CMV strains that currently occur in Bulgaria. Fifty-one isolates and strains found in different regions and various crops were biologically characterized and serologically differentiated into serogroups I and II using different variants of enzyme-linked immunosorbent assay (ELISA) [double antibody sandwich -DAS, antigen coated plate (ACP), triple antibody sandwich (TAS) with poly and monoclonal antibodies] and immunodifusion tests. The ELISA modifications with monoclonal antibodies individually (ACP) or in combination with polyclonal antibodies(TAS-ELISA) are suitable for mass screening of CMV isolates. The results obtained correlated with the polymerase chaine reaction and restriction fragment length polymorphism data reported by other authors. The majority of the isolates belonged to subgroup I, wheres 10, mainly from tomato and pepper, belonged to subgroup II isolates came from the north of Bulgaria.
ISSN0931-1785
Descriptorscucumber mosaic virus
subgroup differentiation
ELISA
VirusesCucumber mosaic virus
VectorsAphididae
PlantsLycopersicon esculentum
Capsicum annuum
Nicotiana tabacum
CropsTomato
Pepper
Tobacco
Cucumber