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Genetic resistances to plant viruses and their vectors

Bibliographic Reference from DPPMA-UNIBA
Department of Plant Protection and Applied Microbiology - UNIBA
Bari - Italy

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Last database update for these data: 2006-12-20 - Data extracted on 2009-04-14 from the database.

Complete nucleotide sequence of Pelargonium zonate spot virus and its relationship with the family Bromoviridae

Bibliographic Reference
Field Value
Document typeJournal article: paper
LanguageEnglish
Year2003
AuthorsFinetti-Sialer, M. M.
Gallitelli, D.
TitleComplete nucleotide sequence of Pelargonium zonate spot virus and its relationship with the family Bromoviridae
SourceJ. Gen. Virol.
Volume84
IssuePt 11
Pages3143-3151
AbstractThe complete sequence of the Pelargonium zonate spot virus (PZSV) genome was determined. It comprises 8477 nt, distributed in three positive-strand RNA species encoding four proteins. RNA-1 is 3383 nt long, with an ORF that encodes a polypeptide with a molecular mass of 108 419 Da (denoted protein 1a). This protein contains the conserved sequence motifs I-III of type I methyltransferases and the seven consensus motifs of the helicases of superfamily 1. RNA-2 is 2435 nt long and encodes a major polypeptide with a molecular mass of 78 944 Da (denoted protein 2a), which shows identity to the RNA-dependent RNA polymerases of positive-strand RNA viruses. RNA-3 is 2659 nt long and contains two major ORFs. The first ORF is located in the 5'portion of the genome and sequence comparison of the putative translation product revealed similarities with the 30K superfamily of virus movement proteins. The second ORF is located in the 3'half and encodes the viral coat protein, which is expressed via a subgenomic RNA, RNA-4. The transcription initiation site of RNA-4 maps to the intergenic region of RNA-3. The organization of the PZSV genome, including the primary structure of terminal non-coding regions, strongly suggests that this virus belongs to the family Bromoviridae. The overall biological and genomic characteristics of PZSV indicate affinities in diverging directions with one or other of the virus species in this family, thus enabling it to be considered as a possible representative of a new genus within the family Bromoviridae.
Web pagePubmed
ISSN0022-1317
ISBN0022-1317 (Print)
Descriptors3'Untranslated Regions chemistry
5'Untranslated Regions chemistry
Base Sequence
Bromoviridae classification and genetics
Molecular Sequence Data
Open Reading Frames
Pelargonium virology
Phylogeny
Viral RNA chemistry