More data about this group.
Last database update for these data: 2006-11-09 - Data extracted on 2009-04-14 from the database.
| Field | Value |
|---|---|
| Document type | Journal article: paper |
| Language | English |
| Year | 2002 |
| Authors | Salmon, M. A. Vendrame, M. Kummert, J. Lepoivre, P. |
| Title | Detection of apple chlorotic leaf spot virus using a 5' nuclease assay with a fluorescent 3' minor groove binder-DNA probe |
| Source | J. Virol. Methods |
| Volume | 104 |
| Issue | 1 |
| Pages | 99-106 |
| Abstract | The development of a real-time 5' nuclease RT-PCR assay for the detection of apple chlorotic leaf spot virus (ACLSV) from infected plant material is described. A short fluorogenic 3' minor groove binder-DNA hydrolysis probe was used to circumvent genome variability between isolates and target a short conserved sequence. The covalent attachment of the minor groove binder moiety at the 3' end of the probe increased the probe/target duplex stability and raised the melting temperature to a range suitable for real-time analysis. The method is rapid, sensitive and takes place within a single tube without post-PCR handling of the amplification products. |
| ISSN | 0166-0934 |
| Descriptors | Apple chlorotic leaf spot virus 5 ' nuclease assay Minor groove binder RT-PCR |
| Viruses | Apple chlorotic leaf spot virus |