More data about this group.
Last database update for these data: 2007-08-29 - Data extracted on 2009-04-14 from the database.
| Field | Value |
|---|---|
| Document type | Journal article: paper |
| Language | English |
| Year | 2001 |
| Authors | Hristova, D. Yordanova, A. |
| Title | Producing of antiserum against a strain from II subgroup of Cucumber mosaic virus(CMV) causing tomato lethal necrosis. |
| Source | Plant Science (Sofia) |
| Volume | 38 |
| Pages | 56-63 |
| Abstract | The Cucumber mosaic virus (CMV) is one of the most wide spread plant viruses in Bulgaria. Our investigations showed that the tomato lethal necrosis is caused by a CMV strains belonging to subgroup II. A CMV strain from II subgroup- 146 D, originated from field tomato, was used for producing the polyclonal antiserum. We used the method of Lot et al., 1974 with some modification of the initial steps of the purification. The purified antigen was injected intramuscularly with increasing concentrations.The total antigen quantity came to 25 mg. The titer of the antiserum against CMV -146 D, detected by ELISA was around 1: 20000 in most of the antiserum samples. In this range of dilution, the relation between the extinction values obtained for the infected plants and these obtained for healthy plants was more than 3, and in some samples it was 6-7. The reaction with the homologous antigen was much more intensive than that with the heterologous antigen (CMV –Uo, I subgroup). |
| ISSN | 0568-465X |
| Descriptors | Antiserum CMV purification |
| Viruses | Cucumber mosaic virus |
| Vectors | Aphididae |
| Plants | Lycopersicon esculentum |
| Crops | Tomato |