More data about this group.
Last database update for these data: 2008-06-04 - Data extracted on 2009-04-14 from the database.
| Field | Value |
|---|---|
| Document type | Journal article: paper |
| Language | English |
| Year | 2003 |
| Authors | Ballut, L. Petit, F. Mouzeyar, S. Le Gall, O. Candresse, T. Schmid, P. Nicolas, P. Badaoui, S. |
| Title | Biochemical identification of proteasome-associated endonuclease activity in sunflower |
| Source | Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology |
| Volume | 1645 |
| Issue | 1 |
| Pages | 30-39 |
| Abstract | Proteasomes have been purified from sunflower hypocotyles. They elute with a molecular mass of 600 kDa from gel filtration columns and two-dimensional gel electrophoresis indicates that the complex contains at least 20 different protein subunits. Peptide microsequencing revealed the presence of four subunits homologous to subunits Beta2, Beta6, Alpha5 and Alpha6 of plant proteasomes. These proteasomes have chymotrypsin-like activity and the highly purified fraction of this complex is associated with an endonuclease activity hydrolyzing Tobacco mosaic virus RNA and Lettuce mosaic virus RNA with a cleavage pattern showing fragments of well-defined size. This is the first evidence of a RNA endonuclease activity associated with plant proteasomes. |
| Web page | Web page Pubmed |
| Descriptors | Amino Acid Sequence Chromatography Gel Chymotrypsin Cysteine Endopeptidases Two-Dimensional Gel Electrophoresis Endonucleases Endoribonucleases Helianthus Molecular Sequence Data Multienzyme Complexes Sequence Alignment |