Last database update for these data: 2006-12-20 - Data extracted on 2009-04-14 from the database.
| No | Title | Method description | Original tool | Offered facilities | Viruses | Crops | Host plants | URL |
|---|---|---|---|---|---|---|---|---|
| 1 | Detection of nine tomato viruses via dot-blot hybridization | A kit was set up, tested and patented for the diagnosis of nine different tomato viruses. The kit includes nine digoxigenin-labeled DNA probes, membranes , controls etc. | Yes | Yes | Cucumber mosaic virus Tomato spotted wilt virus Tomato yellow leaf curl virus Tomato yellow leaf curl Sardinia virus Tomato mosaic virus Alfalfa mosaic virus Pelargonium Zonate Spot Virus Potato virus Y Potato virus X Pepino mosaic virus |
Tomato | Lycopersicon esculentum | Specific Web page |
| 2 | Detection of vegetable viruses via dot-blot hybridization | Developed a protocol for alkaline extraction and dot-blotting on membrane of viral nucleic acids in leaf tissues. Tens of different nucleic acid probes availabe for vegetable viruses (some listed) | Yes | Yes | Artichoke mottled crinkle virus Artichoke Italian latent virus Artichoke latent virus Bean yellow mosaic virus Broad bean wilt virus 1 Broad bean wilt virus 2 Turnip mosaic virus Lettuce mosaic virus Celery mosaic virus Tobacco etch virus Plum pox virus Watermelon mosaic virus Zucchini yellow mosaic virus Cucumber green mottle mosaic virus Impatiens necrotic spot virus Pepper mild mosaic virus Tobacco rattle virus Tomato chlorosis virus Tomato infectious chlorosis virus Eggplant mosaic virus |
Artichoke Lettuce Celery Squash Cucumber Watermelon |
||
| 3 | Detection of plant viruses by RT-PCR | Developed a protocol for RNA extraction and RT-PCR amplification of plant virus-specific DNA fragments. Tens of primer pairs presently in use for vegetable viruses | Yes | Yes |
| No | Germplasm collection | Title | Period | Description | Recordable data | Key words | Viruses | Host plants | Crops | Genetic resistances |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | DPPMA-UNIBA wild Solanum spp. accessions | Tolerance to CMV/satRNA-induced necrosis and stunting in wild Solanum spp. accessions | 2002 and after | Some wild Solanum spp. accessions are tolerant to CMV/satRNA combinations that are aggressive on cultivated tomato | Yes | CMV SatRNA Lethal necrosis Top stunting Tolerance Solanum lycopersicum Solanum spp. |
Cucumber mosaic virus | Lycopersicon esculentum Lycopersicon hirsutum Lycopersicon pennellii Lycopersicon chilense Lycopersicon esculentum var. cerasiforme |
Tomato | tolerance to CMV/satRNA-induced necrosis and stunting |
| No | Category | Name of collection | Persons to contact | Description | Address | Postal code | City | Country | Phone | Plants | Size | Viruses | Size | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Viruses | DPPMA-UNIBA plant virus isolates collection | Donato Gallitelli Fabrizio Cillo |
Dipartimento Protezione delle Piante e Microbiologia Applicata Via Amendola 165/A |
70126 | Bari | Italy | +3908 05 44 30 71 | gallitel@agr.uniba.it | Cucumber mosaic virus Alfalfa mosaic virus Brome mosaic virus Peanut stunt virus Pelargonium Zonate Spot Virus Potato virus Y Watermelon mosaic virus Zucchini yellow mosaic virus Artichoke latent virus Lettuce mosaic virus Turnip mosaic virus Plum pox virus Tomato spotted wilt virus Impatiens necrotic spot virus Artichoke mottled crinkle virus Tomato bushy stunt virus Artichoke Italian latent virus |
5 5 5 5 5 3 3 3 3 3 3 3 5 5 yes yes yes |
|||
| 2 | Plants | DPPMA-UNIBA virus-resistant transgenic lines | Fabrizio Cillo | Transgenic lines expressing viral or non-viral sequences for resistance to viruses | Dipartimento Protezione delle Piante e Microbiologia Applicata Via Amendola 165/A |
70126 | Bari | Italy | +3908 05 44 31 09 | f.cillo@ba.ivv.cnr.it | Lycopersicon esculentum Nicotiana tabacum Nicotiana benthamiana |
30 yes yes |
||
| 3 | Plants | DPPMA-UNIBA wild Solanum spp. accessions | Fabrizio Cillo | A collection of wild tomato relatives (Solanum spp.) from TGRC (tgrc.ucdavis.edu) tested for resistance to CMV/satRNA | Dipartimento Protezione delle Piante e Microbiologia Applicata Via Amendola 165/A |
70126 | Bari | Italy | +3908 05 44 31 09 | f.cillo@ba.ivv.cnr.it | Lycopersicon esculentum Lycopersicon hirsutum Lycopersicon pennellii Lycopersicon chilense Lycopersicon peruvianum Lycopersicon esculentum var. cerasiforme |
25 yes yes yes yes yes |
| No | Resource names | Plant species | Resource type | Origin | Description | Availability | Key words | Viruses | Crops |
|---|---|---|---|---|---|---|---|---|---|
| 1 | UC.TC5 | Lycopersicon esculentum | GMP | tomato cv. UC82 | tomato cv. UC82 expressing a benign variant of CMV satellite RNA (Tfn-satRNA) | please contact us | virus-resistant transgenic plant CMV satellite RNA post-transcriptional gene silencing pathogen-derived resistance PDR |
Cucumber mosaic virus | Tomato |
| 2 | UC.35SPAC | Lycopersicon esculentum | GMP | tomato cv. UC82 | tomato cv. UC82 expressing the PAC gene from Schizosaccharomyces pombe | please contact us | virus-resistant transgenic plant non-pathogen derived resistance |
Cucumber mosaic virus Potato virus Y |
Tomato |
| 3 | UC.S1a UC.S2a UC.S1aS2a |
Lycopersicon esculentum | GMP | tomato cv. UC82 | tomato cv. UC82 expressing the 1a and 2a genes of Cucumber mosaic virus strain S, either alone or together after crossing S1a x S2a | please contact us | CMV replicase | Cucumber mosaic virus | Tomato |
| 4 | Solanum lycopersicum x S. chilense early introgression lines | Lycopersicon esculentum | Population : BC1 (F2) | BC1 population from JW Scott, University of Florida various tomato varieties crosses with S. chilense acc. LA1930, LA1932 |
These tomato lines with introgressions from virus resistant S. chilense accessions served for genetic analysis of resistance to different viruses including ToMoV, TSWV, CMV | no | Tomato mottle virus Tomato spotted wilt virus Cucumber mosaic virus |
Tomato |