Last database update for these data: 2008-06-06 - Data extracted on 2009-04-14 from the database.
| No | Title | Participants | Period | Description | Crops | Viruses | Host plants | Funding |
|---|---|---|---|---|---|---|---|---|
| 1 | Construction of environmentally safe plant virus vectors | Erkki Truve (Leader) | 2004 - 2007 | We propose: i) to construct a new plant virus vector based on cocksfoot mottle sobemovirus (CfMV, an efficiently propagating virus with icosahedral particles which infects monocots growing in temperate pastures); ii) to insert strong antigenic determinants from animal pathogens into the constructed vector; iii) to immunise mice with CfMV-infected plant material; iv) to compare the efficiency of vaccines expressed from differently modified CfMV-based vectors; v) to test the potential of sobemoviruses to recombine in monocot hosts and map the hotspots of recombination; vi) to mutate the possible recombination hotspots in CfMV-based vector; vii) to test the modified CfMV-based vector for potential recombinations with naturally occurring sobemoviruses; viii) to screen Estonian fields and pastures for the possible occurrence of CfMV-related sobemoviruses. |
Oats Barley Rice |
Cocksfoot mottle virus Ryegrass mottle virus Rice yellow mottle virus |
Avena Hordeum vulgare Oryza sativa |
Estonian Science Foundation |
| 2 | Post-transcriptional gene silencing and its suppressors | Erkki Truve (Leader) | 2004 - 2007 | The first objective of the proposed project is to characterize viral suppressors of PTGS from plant virus genus Sobemovirus and to a lesser extent from genus Potyvirus. We plan also to analyse other functions of these named suppressors during the virus life cycle. The second objective of the project is the identification of host factors required for PTGS. For that we plan to exploit the molecular approach by using bacterial and yeast two hybrid systems, co-immunoprecipitations (IPs) and other techniques to identify host components interacting with the suppressors of PTGS. Besides these genome-wide screening approaches it is planned to focus more specifically on the potential role in gene silencing of some plant proteins homologous to animal RNA-degrading 2-5A system |
Cocksfoot mottle virus Rice yellow mottle virus Potato virus Y Tomato bushy stunt virus Potato virus X Cucumber mosaic virus African Cassava mosaic virus Tobacco mosaic virus Tobacco ringspot virus |
Nicotiana tabacum Nicotiana benthamiana |
Estonian Science Foundation | |
| 3 | Expression and function of plant and plant virus genes | Erkki Truve (Leader) | 2003 - 2007 | The main task of the project is to describe the mechanisms of gene silencing in higher plants and clarify the role of viruses in the process. First of all, we plan to identify plant genes which products interact with viral gene silencing suppressors. As gene silencing is studied using viral proteins, the second task of the project is to identify the biological functions of cocksfoot mottle sobemovirus (CfMV) genes and to describe the mechanisms how this particular virus affects antiviral gene silencing mechanisms in host plant. We also plan to elaborate a novel virus-induced gene silencing vector for cereals, based on the infectious cDNA clone of CfMV. In parallel with studies on sobemoviruses and gene silencing we shall study also the role of potato A potyvirus proteins P1 and HC-Pro in this novel mechanism of gene expression regulation. Besides focusing on the studies on gene silencing mechanisms, we foresee also the application of gene silencing for the functional analysis plant genes (RNase L inhibitor) as well as gene families (myosin and myosin-like genes). |
Oats Barley Rice |
Cocksfoot mottle virus Rice yellow mottle virus Potato virus Y Tomato bushy stunt virus Potato virus X Cucumber mosaic virus African Cassava mosaic virus Tobacco mosaic virus Tobacco ringspot virus Ryegrass mottle virus |
Avena Hordeum vulgare Oryza sativa Nicotiana tabacum Nicotiana benthamiana Arabidopsis thaliana |
Estonian Ministry of Education and Research |