More data about this group.
Last database update for these data: 2006-12-20 - Data extracted on 2009-04-14 from the database.
| Field | Value |
|---|---|
| Title | Characterization Of Synergy Between Cucumber Mosaic Virus And Potato Virus Y In Tomato. |
| Period | 2002 and after |
| Description | We investigated on some biological and molecular characteristics of the synergistic effects shown by Cucumber mosaic virus (CMV) and Potato virus Y (PVY) on tomato. Plants inoculated with CMV-Fny exhibited the typical leaf reduction symptoms. We generated two pseudorecombinant strains with different pathogenetic properties inoculating CMV-Fny RNA1 and RNA3 in addition to, respectively, CMV-LS RNA2 (CMV-FLF) and the CMV-Fny RNA2 mutant 209m3D2b (CMV-D2b). CMV-FLF, like the RNA2 donor strain CMV-LS, induced very mild symptoms on tomato, whereas CMV-D2b, a modified CMV-Fny that cannot translate the 2b protein, did not infect tomato systemically. Tomato plants infected with PVY-SON41 showed symptomless infection. CMV-D2b spread systemically when inoculated on PVY-infected tomato plants, revealing that the loss of movement functions of the 2b protein mutant was complemented by PVY analogue functions, as shown also by electron microscopy and immunogold labeling. With the exception of PVY + CMV-D2b, which induced a mild phenotype, symptoms resulted exacerbated in mixed infections, confirming a role for the 2b protein as an enhancer of symptom severity. Symptom severity in mixed infections correlated with increased viral spread and RNA accumulation levels more evident at 60 days post-infection, both in the case of CMV and PVY, although especially the latter virus showed heterogeneity in the distribution throughout the plant and sample-to-sample variation. Small interfering RNA (siRNA) accumulation, the typical hallmark of RNA silencing in virus-infected plants, varied in different PVY/CMV combinations showing positive correlation with the corresponding viral RNA levels, and suggested no role of CMV and PVY RNA silencing suppressors, 2b and HC-Pro proteins respectively, in preventing RNA silencing directed against both viruses in natural infections. |
| Project(s) | 1. CMV- and PVY-mediated RNA silencing in mixed infections in tomato |
| Viruses | Cucumber mosaic virus Potato virus Y |
| Plants | Lycopersicon esculentum |
| Crops | Tomato |