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Results from the ResistVir database
Genetic resistances to plant viruses and their vectors

List of the research results recorded on 2008-10-16

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Research results - 2008-10-16
No Title                                             Group name                Country Participants Period Description                                             Project title                                        Crops Viruses Vectors Host plants Resistances Key words
1 Induction of coat-protein mediated resistance to Arabis Mosaic Virus Plant Biotechnology Unit IAM - IAM-BOKU - BOKU University Austria 1991 - 1992 Biotechnological approaches to the induction of Arabis Mosaic Virus Resistance Arabis mosaic virus Nicotiana benthamiana
2 Transgenic approaches to Sharka resistance Plant Biotechnology Unit IAM - IAM-BOKU - BOKU University Austria 1991 - 1994 Detection du Virus de la Sharka et lutte genetique contre la maladie chez les Prunus
http://www.boku.ac.at/iam/pbiotech/rp5.htm
Plum pox virus Prunus
3 Virus resistance breeding in grapevine Plant Biotechnology Unit IAM - IAM-BOKU - BOKU University Austria 1993 - 2005 Biotechnological methods for resistance breeding in grapevines
http://www.boku.ac.at/iam/pbiotech/rp6.htm
Grapes Grapevine fanleaf virus
Arabis mosaic virus
Grapevine virus A
Grapevine virus B
Vitis
4 Safety assessment of transgenic woody plants Plant Biotechnology Unit IAM - IAM-BOKU - BOKU University Austria 1996 - 1999 Risk assessment with genetically engineered woody plants expressing virus coat protein gene
http://www.boku.ac.at/iam/pbiotech/rp7.htm
Grapes Plum pox virus
Grapevine fanleaf virus
Arabis mosaic virus
Grapevine virus A
Grapevine virus B
Prunus
Vitis
5 Risk assessment with genetically engineered woody plants expressing a virus coat protein gene and marker genes Plant Biotechnology Unit IAM - IAM-BOKU - BOKU University Austria 2001 - 2005 http://www.boku.ac.at/sicherheitsforschung/open-e.htm Characterisation of transgenic fruit trees and analyses of direct and indirect biological interactions Apricot Plum pox virus Prunus armeniaca
6 Identification of tomato varieties with partial resistance to TYLCV Plant Protection Section - ARICY Cyprus 1990 - 2000 Tomato Tomato yellow leaf curl virus Lycopersicon esculentum
7 Resistance to CTV Plant Protection Section - ARICY Cyprus 1992 to present Identification of citrus rootstocks resistant to CTV Citrus tristeza virus
8 Determination of sources of resistance to Plum pox virus in apricots and peaches RICP Department of Virology - Division of Plant Medicine - RICP Czech Republic 1991 - 1996 Cultivar ´Harlayne´was found immune to PPV, cvs. ´Marii de Cenad´, ´Leronda´ and ´Stark Early Orange´ resistant, and cvs. ´Harcot´, and cv. ´Sundrop´ medium resistant. No peach cultivar showed resistance to PPV. Apricot Plum pox virus Prunus armeniaca
9 Determination of the mode of inheritance for resistance to Plum pox virus in apricot RICP Department of Virology - Division of Plant Medicine - RICP Czech Republic 1998 and after Resistance to PPV in apricot is controlled by at least three independent nuclear complementary genes. Apricot Plum pox virus Prunus armeniaca
10 Determination of the degree of resistance of summer squash and Cucurbita moschata to the highly pathogenic Czech ZYMV isolate RICP Department of Virology - Division of Plant Medicine - RICP Czech Republic 2001 - 2004 The U.S. Summer squash cultivar ´Jaguar´ showed the highest degree of resistance to ZYMV. Cucurbita moschata cv. ´Menina´ was immune to ZYMV. Squash
Pumpkins
Zucchini yellow mosaic virus Cucurbita pepo
Cucurbita moschata
11 Safety assessment of transgenic grapevines expressing viral coat protein genes Colmar INRA Virology and vectors laboratory - UMR 1131 - INRA Colmar research center France Emmanuelle Vigne 2002 - 2005 See the paper of Fuchs et al., 2007, Safety assessment of transgenic plums and grapevines expressing viral coat protein genes: New insights into real environmental impact of perennial plants engineered for virus resistance. Journal of Plant Pathology 89 (1), 5-12
Leader: Marc Fuchs
Transvir QLK3-CT-2002-02140 Grapes Grapevine fanleaf virus Xiphinema index Vitis vinifera 1. transgenic resistance
12 Molecular cloning of the pepper recessive resistance gene pvr2 for resistance to potyviruses Functional characterization of plant-pathogen interactions - GAFL - GAP - INRA France Carole Caranta (Leader)
Alain Palloix
Christophe Robaglia
1999 and after A natural recessive resistance gene against potyviruses in pepper was demonstrated to correspond to the eukaryotic translation initiation factor eIF4E. This study corresponds to the molecular characterization of the first natural recessive resistance gene against a plant virus. Pepper Potato virus Y
Tobacco etch virus
Capsicum pvr2 - pepper 1. translation initiation factor eIF4E
13 The pepper pvr6 locus corresponds to a eIF(iso)4E gene Functional characterization of plant-pathogen interactions - GAFL - GAP - INRA France Carole Caranta (Leader) 2001 and after Simultaneous mutations in translation initiation factor eIF4E and eIF(iso)4E are required to prevent Pepper veinal mottle virus infection in pepper. This study provides new data about the recruitment of specific cap-binding isoforms by potyviruses to achieve plant infection. Pepper Pepper veinal mottle virus Capsicum pvr6 - pepper 1. translation initiation factors
14 Molecular mapping of factors for resistance to viruses in the solanaceae and cucurbitaceae Functional characterization of plant-pathogen interactions - GAFL - GAP - INRA France Carole Caranta (Leader)
Catherine Dogimont (Leader)
Alain Palloix
Michel Pitrat
1990 and after Mapping of major genes or quantitative trait loci for complete or partial resistance to economically important viruses in pepper, tomato and melon Potyvirus
Cucumber mosaic virus
Melon necrotic spot virus
Cucurbit aphid-borne yellows virus
Alstroemeria mosaic virus
Capsicum
Lycopersicon esculentum
Cucumis melo
15 Molecular cloning of the Vat gene for resistance to Aphis gossypii in melon Functional characterization of plant-pathogen interactions - GAFL - GAP - INRA France Catherine Dogimont (Leader)
Abdelhafid Bendahmane
Michel Pitrat
1997 and after Aphis gossypii Cucumis melo Vat - muskmelon
16 Metabolomic characterization of constitutive and induced resistance to Myzus persicae confered by the Rm2 gene in peach Functional characterization of plant-pathogen interactions - GAFL - GAP - INRA France Jean-Luc Poëssel (Leader)
Marie-Hélène Sauge
Annick Moing
Thierry Pascal
Claire Dufour
Catherine Deborde
Marie-Noelle Corre
2006 and before Peach Myzus persicae Prunus persica Rm2 - peach
17 Characterization of phagorepellent and toxic effects on Myzus persicae of isolated secondary metabolites identified in resistant peach genotypes Functional characterization of plant-pathogen interactions - GAFL - GAP - INRA France Jean-Luc Poëssel (Leader)
Marie-Hélène Sauge (Leader)
Yves Rahbé
Catherine Deborde
Marie-Noelle Corre
2006 and before Peach Myzus persicae Prunus persica Rm2 - peach
18 Identification and mutation analysis of PVY virulence determinant towards the pvr2 and pot-1 resistance alleles in pepper and tomato. Plant Pathology Unit - SPE - INRA France Benoît Moury (Leader)
Mireille Jacquemond (Leader)
Valérie Ayme
Caroline Morel
Alain Palloix
I. Elisabeth Johansen
1999 and after The PVY viral genome-linked (VPg) protein was shown to be the virulence factor corresponding to the pvr2 resistance alleles in pepper and the pot-1 resistance gene in Lycopersicon hirsutum, which encode eukaryotic translation initiation factors eIF4E. Pepper
Tomato
Potato virus Y
Tobacco etch virus
Capsicum annuum
Lycopersicon hirsutum
pvr2 - pepper
pot-1 - tomato
19 Analysis of the selective pressure exerted on plant virus genomes identifies major epidemiological constraints. Plant Pathology Unit - SPE - INRA France Benoît Moury (Leader) 2000 and after The identification of amino acids in plant virus genomes which undergo positive selection allows to understand the role of epidemiological constraints exerted on virus populations (virulence selection, selection for transmission efficiency by vectors, internal vs. external constraints). Pepper
Potato
Sugar beet
Potato virus Y
Potato virus A
Cucumber mosaic virus
Beet necrotic yellow vein virus
Capsicum
Solanum tuberosum
Beta vulgaris
pvr2 - pepper
20 Identification of the protein encoded by the mo1 gene which confers resistance to LMV in lettuce Plant/Virus Interactions - UMR GDPP - Research Centre INRA Bordeaux-Aquitaine - INRA France Olivier Le Gall (Leader)
Sylvie German-Retana
2000 - 2003 Lettuce Lettuce mosaic virus Lactuca sativa mo1 - lettuce
21 The restriction of the long distance movement of LMV and PPV are controlled by the RTM genes in Arabidopsis thaliana Plant/Virus Interactions - UMR GDPP - Research Centre INRA Bordeaux-Aquitaine - INRA France Frédéric Revers (Leader)
Véronique Decroocq
2003 - 2005 Lettuce mosaic virus
Plum pox virus
Arabidopsis thaliana RTM - Arabidopsis thaliana
22 The translation initiation factor eIF4G and its isoformes are used by potyviruses to achieve their viral cycle and are potentially new target to developp resistance Plant/Virus Interactions - UMR GDPP - Research Centre INRA Bordeaux-Aquitaine - INRA France Sylvie German-Retana (Leader) 2003 - 2006 Lettuce mosaic virus
Plum pox virus
Turnip mosaic virus
Arabidopsis thaliana
23 Analogues of virus resistance genes map to QTLs for resistance to sharka disease in Prunus Plant/Virus Interactions - UMR GDPP - Research Centre INRA Bordeaux-Aquitaine - INRA France Véronique Decroocq (Leader)
Valérie Schurdi-Levraud
2000 - 2004 Apricot
Peaches and nectarines
Plums and sloes
Plum pox virus Prunus
24 Development of a method (NEWeB) for studying interactions of host or viral proteins with virus particles. PPIP Plant Pathology Laboratory - PPIP - NAGREF Greece Ioannis N. Manoussopoulos (Leader)
M. Tsagris (Leader)
E. Maiss (Leader)
1995 - 1997 Determination of the PPV virus particle-HC interaction. Plum pox virus Nicotiana benthamiana
Nicotiana clevelandii
25 Investigation on the distribution of Lettuce big vein virus and Mirafiori lettuce virus in lettuce crops in Greece PPIP Plant Pathology Laboratory - PPIP - NAGREF Greece Ioannis N. Manoussopoulos (Leader)
P. Roggero (Leader)
2001 - 2003 Detection of LBVV and MiLV under natural conditions and assesment on the effect on lettuce quality Lettuce Mirafiori lettuce virus
Lettuce big-vein associated virus
Lactuca sativa
26 Transgenic cucumber plants resistant to several viruses ARO Department of Plant Pathology - Institute of Plant Protection - ARO Israel Amit Gal-On (Leader)
Aaron Zelcer
Yeheskal Antignus
Victor Gaba
Dalia Wolf
Diana Liebman
2000 - 2006 We generated in homozygote commercial cucumber resistant to several important viruses: resistance was verified by various inoculation methods in the greenhouse Cucumber Zucchini yellow mosaic virus
Cucumber fruit mottle mosaic virus
Cucumber vein yellowing virus
Cucurbit yellow stunting disorder virus
Cucumis sativus 1. transgenic resistance
27 Expression of protein and peptides via attenuated virus vector ARO Department of Plant Pathology - Institute of Plant Protection - ARO Israel Amit Gal-On (Leader) 1998 - 2006 We expressed many genes via ZYMV vector: some genes benefitial to agriculture were field tested, and some highly valued proteins were partially purified from the plant. We also succeeded to use this vector for epitope presentation to raise specific antibodies in chicks. We are now studying the use of this vector for gene silencing. Musk melon
Squash
Zucchini yellow mosaic virus Cucumis melo
Citrullus lanatus
1. ZYMV-AG
28 Potyvirus resistance ARO Department of Plant Pathology - Institute of Plant Protection - ARO Israel Victor Gaba (Leader)
Amit Gal-On
Arieh Rosner
2003 - 2006 We have produced a transgenic tobacco line with a 600bp inverted repeat from the PVY replicase gene that is very resistant to the homologous virus, and sequence-related virus cultures. The resistance is strong against less related PVY strains, but breaks eventually. Tobacco Potato virus Y Nicotiana tabacum
29 Helper component HC-Pro as a key potyvirus symptom expression determinant ARO Department of Plant Pathology - Institute of Plant Protection - ARO Israel Amit Gal-On (Leader) 2003 - 2006 Potyvirus
Zucchini yellow mosaic virus
Potato virus Y
Turnip mosaic virus
Papaya ringspot virus
Cucurbitaceae
Nicotiana benthamiana
Arabidopsis thaliana
30 Production of transgenic plants resistant to Plum pox virus by RNAi technology C.R.A.-Plant Pathology Research Institute - CRA Italy Vincenza Ilardi (Leader)
Elisa Di Nicola-Negri
Angela Brunetti
2001 - 2004 Production of transgenic plants resistant to PPV Plum pox virus Nicotiana benthamiana 1. RNA silencing
2. 5' end
3. P1
4. HC-Pro
5. P3
31 Production of transgenic tomato plants resistant to Cucumber mosaic virus by coat protein strategy C.R.A.-Plant Pathology Research Institute - CRA Italy Marina Barba (Leader)
Vincenza Ilardi
Laura Tomassoli
Wojciech Kaniewski
1992 - 1997 Tomato Cucumber mosaic virus Lycopersicon esculentum 1. coat protein
2. transgenic
3. CMV
4. Resistance
32 Use of wild accessions and near isogenic lines of Lycopersicon hirsutum for the identification of host factors involved in the susceptibility to Cucumber mosaic virus and its satellite RNA. Department of Plant Protection and Applied Microbiology - UNIBA Italy 2002 and after Several accessions of wild and cultivated species of the genus Lycopersicon, including L. esculentum (tomato), L. hirsutum, L. chilense, L. pimpinellifolium, L. pennellii and L. peruvianum, were tested for their susceptibility to combinations of Cucumber mosaic virus (CMV) with variants of satellite RNA (satRNA) co-inducing diverse disease phenotypes in tomato. The following CMV preparations have been used for the characterisation of Lycopersicon spp./CMV/satRNA interactions: a) CMV-Fny (no satRNA); b) CMV-FB (Fny + a Benign satRNA variant, symptomless on tomato); c) CMV-FS (Fny + a tomato Stunting-inducing satRNA variant); d) CMV-FN (Fny + a tomato Necrosis-inducing satRNA). CMV-Fny induced on all hosts the typical leaf malformation (“shoestring”, reduced leaflet blade), and no resistance was observed on any of the tested genotypes. CMV-FB induced on all hosts a phenotype characterised by latent infection accompanied by the down-regulation of viral RNA replication, referred to as LIDR (Latent Infection, Down-Regulation). Interestingly, LIDR was observed also on some wild Lycopersicon accessions inoculated with CMV-FS and CMV-FN that are aggressive on tomato. In particular, accessions of L. chilense showed LIDR with both the aggressive CMV/satRNA combinations tested, whereas accessions of L. hirsutum and L. pennellii showed LIDR upon inoculation with CMV-FS, but susceptibility to systemic necrosis induced by CMV-FN. A screening of 99 near isogenic lines, containing single introgressions from L. hirsutum accession LA1777 in the L. esculentum cv. E6206 genetic background (Monforte and Tanksley, Genome/Génome 43: 803, 2000), was undertaken to map host factors determining differential susceptibility to CMV/satRNA in Lycopersicon spp. Observing a temporary resistance to CMV-FS on one of these lines, a possible host factor interfering with stunting, but not with systemic necrosis, was mapped genetically to L. hirsutum chromosome 6. Other loci were identified, correlating with changes in symptoms expression rather than with absence of symptoms. From these preliminary studies the following conclusions are drawn: a) CMV/satRNAs diverse symptoms (leaf malformation, stunting, necrosis) on host Lycopersicon spp. are due to alterations of apparently individual and uncoupled pathways; b) L. chilense can be a source of gene(s) responsible for protection from CMV/satRNA-induced lethal necrosis; c) the LIDR phenotype appears to be regulated by a multigenic character not directly transferable to cultivated tomato genotypes. Molecular and genetic bases involved in the susceptibility of Lycopersicon spp. to cucumber mosaic virus and its satellite RNA Tomato Cucumber mosaic virus Lycopersicon esculentum
33 Characterization Of Synergy Between Cucumber Mosaic Virus And Potato Virus Y In Tomato. Department of Plant Protection and Applied Microbiology - UNIBA Italy 2002 and after We investigated on some biological and molecular characteristics of the synergistic effects shown by Cucumber mosaic virus (CMV) and Potato virus Y (PVY) on tomato. Plants inoculated with CMV-Fny exhibited the typical leaf reduction symptoms. We generated two pseudorecombinant strains with different pathogenetic properties inoculating CMV-Fny RNA1 and RNA3 in addition to, respectively, CMV-LS RNA2 (CMV-FLF) and the CMV-Fny RNA2 mutant 209m3D2b (CMV-D2b). CMV-FLF, like the RNA2 donor strain CMV-LS, induced very mild symptoms on tomato, whereas CMV-D2b, a modified CMV-Fny that cannot translate the 2b protein, did not infect tomato systemically. Tomato plants infected with PVY-SON41 showed symptomless infection. CMV-D2b spread systemically when inoculated on PVY-infected tomato plants, revealing that the loss of movement functions of the 2b protein mutant was complemented by PVY analogue functions, as shown also by electron microscopy and immunogold labeling. With the exception of PVY + CMV-D2b, which induced a mild phenotype, symptoms resulted exacerbated in mixed infections, confirming a role for the 2b protein as an enhancer of symptom severity. Symptom severity in mixed infections correlated with increased viral spread and RNA accumulation levels more evident at 60 days post-infection, both in the case of CMV and PVY, although especially the latter virus showed heterogeneity in the distribution throughout the plant and sample-to-sample variation. Small interfering RNA (siRNA) accumulation, the typical hallmark of RNA silencing in virus-infected plants, varied in different PVY/CMV combinations showing positive correlation with the corresponding viral RNA levels, and suggested no role of CMV and PVY RNA silencing suppressors, 2b and HC-Pro proteins respectively, in preventing RNA silencing directed against both viruses in natural infections. CMV- and PVY-mediated RNA silencing in mixed infections in tomato Tomato Cucumber mosaic virus
Potato virus Y
Lycopersicon esculentum
34 Transgenic lines expressing mutant versions of the Rep protein of TYLCSV resistant to virus infection Institute of Plant Virology - CNR Italy Emanuela Noris (Leader) 2000 - 2006 Transgenic and natural resistance to virus and phytoplasma diseases of crops Tomato Tomato yellow leaf curl virus Lycopersicon esculentum
Nicotiana benthamiana
1. TYLCSV
35 Characterization of the resistance mediated by the Cucumis melo nsv gene. Plant Pathology Group - Stress Biology and Plant Pathology Department - CEBAS - CSIC Spain Miguel A. Aranda (Leader)
Verónica Truniger
Cristina Nieto
2000 and after Musk melon Melon necrotic spot virus Cucumis melo
36 Detection of Turkish Beet Necrotic Yellow Vein Benyvirus (BNYVV) Isolates by RT-PCR (Plant Pathologyin press)
Occurence of RNA 5 in Turkish BNYVV Isolates. (Phytoparasitica, in press)
Department of Plant Protection AU - AUZF - AU Turkey Filiz ERTUNÇ (Leader)
Digdem ILHAN
1999 - 2003 Şeker Pancarı Nekrotik Sarı Damar Virusunun Genomik Konformasyonunun RT-PCR Yöntemi ile Belirlenmesi. Sugar beet Beet necrotic yellow vein virus Polymyxa betae Beta vulgaris
37 The occurence and molecular characterization of PNRSV isolates in Turkey. Department of Plant Protection AU - AUZF - AU Turkey Cigdem ULUBAS 2002 - 2004 Vişne ve Kirazda Enfeksiyon Yapan Virüslerin RT-PCR Yöntemi ile Saptanması ve Karakterizasyonu. Molecular Characterization and detection of sour and sweet cherry viruses by RT-PCR Method. Cherry (all varieties) Prunus necrotic ringspot virus Prunus
38 Identification of markers tightly linked to sbm recessive genes for resistance to Pea seed borne mosaic virus John Innes Centre - BBSRC United Kingdom Andy Maule (Leader) 2001 - 2004 mapping of linked markers to sbm-1/sbm-4 eIF4E-based recessive resistance against pea seed borne mosaic potyvirus in pea Peas Pea seed-borne mosaic virus Pisum sativum sbm-1 - Pisum sativum
39 The potyvirus recessive resistance gene, sbm1, identifies a novel role for translation initiation factor eIF4e in cell-to-cell trafficking. Sbm-1 and Sbm-4 shown to be distinct allelic variants of eIF4E. John Innes Centre - BBSRC United Kingdom Andy Maule (Leader) 2001 - 2004 Functional study of the role of eIF4E eIF4E-based recessive resistance against pea seed borne mosaic potyvirus in pea Peas Pea seed-borne mosaic virus Pisum sativum sbm-1 - Pisum sativum 1. potyvirus
2. recessive resistance
3. eIF4E
4. cell-to-cell movement
40 Resistance to Maize streak virus localises to the distal region of chromosome 6 in barley John Innes Centre - BBSRC United Kingdom Margaret Irene Boulton (Leader)
David Laurie
2002 - 2006 mapping of HvMsv-1 Biological and genetic characterisation of novel resistance to Maize streak virus in barley Barley Maize streak virus Hordeum vulgare HvMsv-1 - barley 1. Maize streak virus
2. resistance barley
3. mapping
41 Resistance to Maize streak virus, conferred by HvMsv-1 is developmentally-regulated John Innes Centre - BBSRC United Kingdom Margaret Irene Boulton (Leader)
David Laurie
2001 - 2006 Biological characterisation of HvMsv-1-mediated resistance Biological and genetic characterisation of novel resistance to Maize streak virus in barley Barley
Maize
Wheat
Rice
Maize streak virus Hordeum vulgare
Zea mays
Triticum aestivum
Oryza sativa
HvMsv-1 - barley 1. Maize streak virus
2. barley
3. developmental resistance
4. host range